知识与财富的链接
目的：观察肝癌细胞抗原致敏的树突状细胞（Dendriticcells,DC）与细胞因子诱导的杀伤细胞（Cytokine-inducedkillercells,CIK）共培养后的细胞增殖活性、表型的变化及其对肝癌细胞HepG2的杀伤活性。方法：采集健康供者的外周血单个核细胞（Peripheralbloodmononuclearcells,PBMNC）,常规诱导出DC、CIK,用肝癌细胞HepG2抗原冲击DC（Ag-DC）,并将其与CIK共培养（Ag-DC-CIK）,观察CIK和Ag-DC-CIK的细胞表型及增殖活性,并以肝癌细胞HepG2作为靶细胞,用MTT法检测CIK和Ag-DC-CIK的杀伤活性。结果：肝癌细胞抗原致敏的DC与CIK细胞共培养后,Ag-DC-CIK细胞群增殖活性明显增强,且高表达CD3+CD8+、CD3+CD56+双阳性细胞,其增殖活性和杀伤活性较单纯的CIK细胞更高（P<0.05）。结论：肿瘤抗原致敏的DC与CIK共培养获得的Ag-DC-CIK增殖活性和对肝癌细胞的杀伤活性显著高于CIK细胞。
Objective：To observe the proliferation activities,phenotype changes and killing activities of liver cell antigen-pulsed den－dritic cells（Ag-DC） cultured with cytokine-induced killer（CIK） cells against HepG2 hepatocarcinoma cells. Methods：Peripheral blood mononuclear cells（PBMNC） were isolated from healthy donors,then DC and CIK cells were induced. The Ag-DC was co-cul－tured with CIK（Ag-DC-CIK） and the phenotype and proliferation of CIK and Ag-DC-CIK cells were observed. Their cytotoxicity ac－tivities against HepG2 hepatocarcinoma cells were detected by MTT assay. Results：The proliferation of Ag-DC-CIK cell group was significantly enhanced and high expressions of double positive cells of CD3+CD8+ and CD3+CD56+ were observed. The proliferation and cytotoxic activity were higher in Ag-DC-CIK cells than in CIK cells（P<0.05）. Conclusion：The proliferation and liver cancer cell-killing activity of Ag-DC-CIK cells which obtained from co-culturing of Ag-DC and CIK cells are significantly higher compared with those of CIK cells.
