The yield stability and adaptability of twelve sugarcane lines in five locations at the sixth national regional trials were studied by using AMMI (additive main effects and multiplicative interaction) biplot method. The results showed that there were significant differences in six yield traits among genotypes and environments, so did the interaction effects between genotype and environment. FN30 and YG16 had high stem yield and sugar content with strong yield stability. However, the overall stability of the two sugarcane lines was not high, which indicated that they should be promoted in a certain region. AMMI model could clearly explain the genotype effect, environment effect and genotype-environment interaction effect on yield trait.