知识与财富的链接
目的:通过观察受孕小鼠孕期给补肾方剂对其子代胎儿宫内发育迟缓（IUGR）模型抗致病性大肠杆菌能力及网状内皮系统（RES）吞噬功能的影响,探讨中医"肾为先天之本,肾主生殖发育"和"补母益子"理论。方法:将同期妊娠小鼠分为正常对照组、IUGR模型组、左归丸组、右归丸组、五子衍宗丸组、四君子汤组,共6组。模型组于孕第7天用40%乙醇灌胃,12 mL·kg-1,1日1次,共14 d,各治疗组分别在造模的同时ig予补肾方剂（五子衍宗丸、左归丸、右归丸）及补气方剂（四君子汤）,30 g·kg-1,30 mL·kg-1,1日1次,共14 d。每窝子鼠2～4只供检测各组子1代小鼠抗致病性大肠杆菌能力及网状内皮系统（RES）吞噬功能。结果:孕鼠于孕7～20 d ig补肾方剂左归丸的子鼠感染致病性大肠杆菌24 h存活率（8只）明显高于IUGR模型组（P<0.05）及正常对照组（P<0.05）;IUGR模型子鼠RES碳粒廓清吞噬指数K（1.15±0.14）及校正吞噬指数α（5.68±2.63）均显著低于正常对照组（2.14±0.14,9.26±1.67,P<0.05）,而补肾方剂左归丸组吞噬指数K及校正吞噬指数α为（2.54±0.12,8.51±1.94）、右归丸组为（0.98±0.07,8.86±1.76）、五子衍宗丸组（2.28±0.09,8.70±1.32）均明显高于模型组（P<0.05）而接近正常组;补气方剂四君子汤对子代的抗细菌感染力及RES吞噬功能未见明显作用。结论:孕鼠于孕7～20 d ig左归丸等补肾方剂,可显著提高IUGR小鼠的免疫力,从免疫学角度为中医"肾为先天之本,肾主生殖发育"和"补母益子"理论提供了实验依据。
Objective: To study the effect of prescription with functuon of tonifying the kidney on the resistance ability against pathogenic escherichia coli and phagocytic function of reticuloendothelial system(RES)in newborn mice with intrauterine growth retardation(IUGR),and to investigate the theory of TCM:’the kidney being the origin of congential constitution and foundation of reproduction and development of an individual’,’invigorating the mother to benefit the children’.Method: The pregnant mice were randomly divided into 6 groups.The control group was fed normally.In model group,the pregnant mice were fed with alcohol on the 7th day,ig,12 mL·kg-1,for 14 days.In other 4 experiment groups,the pregnant mice were fed respectively by Zuogui Wan(ZGW,a prescription for reinforcing kidney Yin) or Yougui Wan(YGW,a prescription for reinforcing kidney Yang)or Wuzi Yanzong Wan(WZYZW,a prescription for reinforcing both of kidney Yang and kidney Yin)or Sijunzi decoction(SJZD,a prescription for supplementing Qi) ig,30 g·kg-1,30 mL·kg-1,for 14 days,at the same time.After birth,the newborn mice were infected with pathogenic escherichia coli,the phagocytic functions of reticuloendothelial system(RES)were detected.Result: In the treated groups,the survival rate of newborn mice infected by pathogenic escherichia coli(8) for 24 hours was higher than that in model group(P<0.05) and control group(P<0.05).The phagocytic function of reticuloendothelial system(RES)in model group(K:1.15±0.14,α:5.68±2.63)was markedly lower than control group(2.14±0.14,9.26±1.67,P<0.05).The phagocytic function in the treated group was markedly higher than that in model group(ZGW:2.54±0.12,8.51±1.94,P<0.05,YGW:0.98±0.07,8.86±1.76,P<0.05,WZYZW:2.28±0.09,8.70±1.32,P<0.05).But same result was not found in for supplementing Qi group.Conclusion: Prescriptions with function of tonifying the kidney like ZGW can enhance immunologic functions in newborn mice with IUGR.And this may provide an evidence for the theory-’the kidney being the origin of congential constitution and foundation of reproduction and development of an individual ’,’invigorating the mother to benefit the children’in TCM from the aspect of immune theory.
