知识与财富的链接
为了解当前青海省普通小麦种质材料醇溶蛋白的遗传多样性,利用酸性聚丙烯酰胺凝胶电泳(A-PAGE)技术对青海省77份普通小麦种质材料进行了醇溶蛋白遗传多样性分析。结果表明,供试材料中共分离出蛋白谱带1 237条,迁移率不同的谱带类型37种,其中迁移率编号为2、19和3号的谱带出现频率最高,分别为98.7%、98.7%和97.4%;3条谱带(15、16和17号)出现频率低于10.5%;其余31条谱带出现频率为19.5%～84.4%。供试材料醇溶蛋白谱带多态性较高,每个材料可电泳分离出11～21条谱带,其中具有14～18条谱带的材料居多。不同材料间的遗传相似系数变化范围为0.55～0.94,说明供试材料具有丰富的遗传多样性。聚类分析将供试材料分成6大类,聚类结果在一定程度上反映了供试材料间的亲缘关系。
In order to investigate the genetic diversity at gliadin loci in wheat (Triticum L.) cultivars and germplasm resources, 77 accessions from Qinghai province in the last 20 years were analyzed by acid polyacrylamide gel electrophoresis(A-PAGE). The results showed that a total of 1 237 protein bands were identified with 37 different relative mobility rates in the examined wheat accessions. The bands of No. 2, 19 and 3 had the highest frequency of 98.7%,98.7%,97.4%, respectively.Three bands（No.15,16 and 17 ） had the frequencies below 10.5%, and the remaining 31 bands had frequency ranged from 19.53%~84.4%, with higher polymorphisms. Each accession contained 11~21 bands, and most of them ranged from 14 to 18. The genetic similarity (GS) based on gliadins was between 0.55 and 0.94, indicating that higher genetic diversity existed among the accessions tested. Cluster analysis divided the accessions into five major classes, which reflected the relationship of these accessions.
