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Expression of superoxide dismutase (SOD) genes from the copper-exposed polychaete, Neanthes succinea

ISSN:0025-326X
2011年第63卷第5-12期
Rhee JS,Won EJ,Kim RO,Lee J,Shin KH,Lee JS Rhee Jae-Sung,Won Eun-Ji,Kim Ryeo-Ok,Lee Jehee,Shin Kyung-Hoon,Lee Jae-Seong

To evaluate the superoxide dismutase (SOD) genes as a potential indicator of environmental stress, we cloned and sequenced the full-length cDNA of a Cu/Zn-SOD and Mn-SOD genes from the marine polychaete, Neanthes succinea. Ns-Cu/Zn- and Mn-SOD expression was measured using quantitative real-time RT-PCR in response to the oxidative stress induced by copper in the form of CuCl2. Ns-Cu/Zn-SOD expression increased in a dose dependent manner but the increase of Mn-SOD expression was evident only at a high concentration of copper. Particularly, Ns-Mn-SOD dramatically increased in the 72 μg/L CuCl2-exposed groups, indicating that both Ns-SOD genes were induced by copper exposure. These results suggest that Ns-Cu/Zn- and Ns-Mn-SOD would play important roles in the detoxification of cellular oxidative stresses in the polychaete, N. succinea. Furthermore, as part of field work, the marine polychaete, N. succineaCu/Zn-SOD mRNA collected from the polluted area was accordingly upregulated in expression but not for NS-Mn-SOD.

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ISSN:0025-326X
2011年第63卷第5-12期

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