知识与财富的链接
目的：建立HPLC同时测定决明子中红镰霉素龙胆二糖苷、决明子苷、决明子苷C含量的方法。方法：采用HPLC,Dionex Acclaim C18柱（4.6 mm×250 mm,5 μm）,流动相乙腈-四氢呋喃-1%冰醋酸（17：2：81）,流速1 mL·min-1,柱温30 ℃,检测波长278 nm。结果：红镰霉素龙胆二糖苷、决明子苷、决明子苷C的进样量分别在0.218~1.090,0.188~0.940,0.200~1.000 μg和峰面积呈良好的线性关系,回归方程分别为Y=54.017X-0.344 6 （r=0.999 9）,Y=78.63X-0.354（r=0.999 9）,Y=74.098X-0.433 5 （r=0.999 9）;平均加样回收率分别为98.43%（RSD 1.75%）,101.89%（RSD 1.10%）,102.46%（RSD 1.15%）。结论：该方法快速、灵敏、准确、可靠、重复性好,可用于中药决明子药材的质量控制。
Objective: To establish a high performance liquid chromatographic method (HPLC) for simultaneous determination of rubrofusarin gentiobioside, casside, cassiaside C in Cassia obtusifolia. Method: The HPLC with Dionex Acclaim C18(4.6 mm×250 mm, 5 μm) column was used, acetonitrile-THF-1% acetic acid (17 ：2 ：81) was used as a mobile phase, with flow rate of 1 mL·min-1, column temperature at 30 ℃ and detection wavelength at 278 nm. Result: Rubrofusarin gentiobioside, casside, cassiaside C showed good linearity (r=0.999 9) in the range of 0.218-1.09, 0.188-0.94, 0.200-1.000 μg, regressive equation were Y=54.017X-0.344 6 (r=0.999 9),Y=78.63X-0.354(r=0.999 9),Y=74.098X-0.433 5 (r=0.999 9)respectively;the average recoveries of the method were 98.43%, 101.89%, 102.46%, RSD were 1.75%, 1.10%, 1.15%. Conclusion: The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.
