知识与财富的链接
本文以香港巨牡蛎(Crassostrea hongkongensis)和太平洋牡蛎(C.gigas)为研究对象,进行牡蛎异源三倍体研究试验。在水温25℃条件下,香港巨牡蛎剥离卵子于海水中浸泡促熟1.5h后,与太平洋牡蛎精子受精,获得最高卵裂率为(24.40±3.79)%。受精后20min,采用盐度为10的低渗海水处理杂交受精卵25min,可获得68.65%的异源三倍体幼虫,孵化率为12.71%。异源三倍体幼虫的存活率和生长率均未表现出优势。受精后第9天,异源三倍体幼虫的存活率仅为(0.116±0.023)%,平均壳长为(103.50±0.85)(m;对照组的存活率和壳长分别为(42.17±2.74)%和(123.25±8.60)μm。幼虫在附着变态前死亡。
Prior to the allotriploid induction,the feasibility of fertilization between Crassostrea hongkongensis(Lam and Morton,2003) eggs and C.gigas(Thunberg,1973) sperm had been verified before.Results showed that cleavage rate of(24.40±3.79) % of the interspecific hybrid can be received with the eggs immersed in 25℃ seawater for 1.5h.The zygotes treated for 25 min with a hypotonic solution of 10 salinities at 20min after fertilization can yield the highest triploidy rate of 68.65%,and the hatchery rate of 12.71%.No statistical advantages of growth rate and survival rate were found in allotriploid group compared with control group.The survival rate and shell length of allotriploid group were(0.116±0.023)% and(103.50±0.85)μm at the 9th day after insemination.However,the survival rate and shell length of control group were(42.17±2.74)% and(123.25±8.60)μm.All allotriploid hybrid larvae died before metamorphism.
