知识与财富的链接
对36个引自加拿大的豌豆品种(系)进行抗白粉病表型和标记基因型鉴定,明确了豌豆品种Cooper和Tara白粉病抗性等位基因。苗期接种了2个不同地理来源的豌豆白粉病菌分离物,32个品种(系)对2个分离物均表现为免疫;品系MP1818-2对云南白粉菌分离物EPYN免疫,但对北京分离物EPBJ感病;其余3个品种对2个分离物均感病。4个与豌豆抗白粉病基因er1连锁的SCAR标记将36个豌豆品种(系)区分为5个标记基因型。与野生型PsMLO1基因序列比较发现,豌豆品种Cooper和Tara的PsMLO1候选基因均在680 bp处发生C变G的单核苷酸突变。
In this study, the phenotype and genotype of powdery mildew resistance in 36 pea cultivars or lines introduced from Canada were identified, and the allele at powdery mildew resistance gene er1 locus in cultivars Cooper and Tara were investigated. All tested cultivars or lines were inoculated by two powdery mildew isolates from different geographical origins in seedling stage, respectively. Thirty-two cultivars or lines were immune to the two isolates, and line MP1818-2 was immune to isolate EPYN and susceptible to isolate EPBJ, whereas 3 cultivars were susceptible to the two isolates. Five marker genotypes were identified in the 36 cultivars or lines by 4 SCAR markers linked to er1. Sequence analysis revealed that both PsMLO1 candidate gene in Cooper and Tara contained a point mutation which G replaced C of PsMLO1 gene at 680 bp site.
