知识与财富的链接
目的：D-半乳糖sc建立亚急性衰老小鼠模型,研究豆丹的抗衰老作用。方法：将实验动物随机分为7组,分别为正常对照组、衰老模型组、西洋参组（0.5 g·kg-1）、豆丹水提液高（2.8 g·kg-1）、低剂量组（1.4 g·kg-1）、豆丹醇提液高（12.5 g·kg-1）、低剂量组（6.25 g·kg-1）。造模14 d后开始给药,给药14 d后测定负重游泳时间、脏器系数、肝脏超氧化物歧化酶（SOD）活性、肝脏及脑组织谷胱甘肽过氧化物酶（GSH-Px）活性、肝及脑组织丙二醛（MDA）含量。结果：与正常组比较,衰老模型组负重游泳时间、脏器指数、SOD及GSH-Px均降低,MDA含量增加,且均有显著性差异（P<0.05或P<0.01）;与衰老模型组比较,豆丹水提液、醇提液均能显著延长负重游泳时间（P<0.05或P<0.01）,提高脏器指数（P<0.05）,提高SOD,GSH-Px活性（P<0.05或P<0.01）,降低MDA含量（P<0.05）,且对雌雄小鼠的作用大体相当。结论：豆丹可能通过提高机体免疫、抗脂质过氧化和清除氧自由基的能力来发挥抗衰老的作用。
Objective: Anti-aging effect of Clanis bilineata tsingtauica was investigated through the model of subacute aging mice induced by subcutaneous injection of D-galactose in the study. Method: The Kunming mice were divided into seven groups:normal control group,aging model group, Panax quinquefolius positive control group,low and high-dose C. bilineata tsingtauica's water extraction group(1.4,2.8 g·kg-1),low and high-dose Clanis bilineata tsingtauica's 75% ethanol extraction (6.25, 12.5 g·kg-1).14 days after the mice were sc given D-galactose, the corresponding drugs were ig given to the mice,respectively.After 14 days, the loaded swimming time, the viscera index,superoxide dismutase(SOD) activities in liver homogenate,glutathione peroxidase(GSH-Px) activities in liver and brain homogenate and malondialdehyde(MDA) content in liver and brain homogenate were measured. Result: Compared with the normal control group, the loaded swimming time, the viscera index,the T-SOD activities and the GSH-Px activities in liver and brain were markedly reduced in aging model group,while the content of MDA was markedly increased(P<0.05 or P<0.01).Both water extraction and 75% ethanl extraction of C. bilineata tsingtauica could significantly extend the loaded swimming time(P<0.05 or P<0.01), increase the viscera index(P<0.05), elevat the activities of T-SOD and GSH-Px and decrease the contents of MDA(P<0.05 or P<0.01),and the effects on male and female are generally the same. Conclusion: C.bilineata tsingtauica has an anti-aging effect by improving immune and antioxidant capacity.
