The paper studied the interlayer oxidation zones and uranium mineralization in the northwest margin of Qaidam Basin, pointed out that the wedge-out of interlayer oxidation zones accompanied with syngenetic faults and anticlinal zones of oil-gas accumulation area in the study region where the primary uranium mineralization type occurred in the junction between penetration water with oxygen and exudation water of oil field, the key strata for uranium prospecting should be focused on Jurassic and Tertiary.