介绍了建立良好植物悬浮细胞系的几个关键技术(外植体的选择、胚性愈伤组织的甄别及合适的培养条件),同时阐明了悬浮细胞系建立过程中存在的一些问题及相应的解决措施.
Several key technologies for establishing an efficient plant cell suspension are described, including se- lection of explants, identification of embryogenic callus and proper culture condition. Some problems and their solutions in plant cell suspension culture are also demonstrated to provide reference for the related research work.