知识与财富的链接
目的 通过检测慢性结石性胆囊炎患者胆囊胆汁胆固醇浓度(biliary cholesterol concentration,BCC)及胆囊黏膜上皮A型胆固醇受体(cholecystokinin-A receptor,CCK-AR)蛋白表达强度,明确BCC与CCK-AR的特点及关系。方法 通过免疫组化的方法检测34例行腹腔镜手术的慢性结石性胆囊炎胆囊黏膜上皮CCK-AR蛋白表达强度,同时用生化方法检测胆囊内胆汁胆固醇浓度,观察结果特点并分析相关性。结果 所有标本胆囊黏膜上皮CCK-AR呈阳性表达,其中13例呈弱阳性表达,9例呈中阳性表达,12例呈强阳性表达。胆囊内胆汁胆固醇浓度在CCK-AR弱阳性组,CCK-AR中阳性组,CCK-AR强阳性组分别为(144±51)mmol/L,(168±41)mmol/L和(96±19)mmol/L,与CCK-AR表达强度呈负相关(r=-0.702,P=0.000)。结论〖HTK〗 胆汁胆固醇浓度可作为评估胆囊黏膜上皮CCK-AR分子蛋白表达的潜在指标。
Objective To investigate the relationship of biliary cholesterol concentration and cholesterol cholecystokinin-A receptor expression in chronic calculous cholecystitis.Methods The biliary cholesterol concentration in the gallbladder of4 cases were tested by biochemistry and cholesterol cholecystokinin-A receptor expression in the gallbladder mucosa of4 cases were detected by immunohistochemistry.Results The cholesterol cholecystokinin-A receptor (CCK-AR) expression in the gallbladder mucosa of4 cases showed positive expression,in which weak positive3 cases,moderate positive 9 cases,and strong positive2 cases.Bile cholesterol concentration in the weak positive group was (144±51) mmol/L,in the moderate positive group was (168±41) mmol/ L,and in the strong positive group was (96±19) mmol/L.The CCK-AR expression and biliary cholesterol concentration showed negative correlation (r=-0.702,P=0.000).Conclusion The bile cholesterol concentration may be used as potential indicators of CCK-AR expression in chronic calculous cholecystitis,providing a theoretical basis for further study of the gallbladder dynamics.
