知识与财富的链接
目的:建立广藿香配方颗粒中咖啡酸、毛蕊花糖苷、异毛蕊花糖苷和广藿香酮的含量测定方法,为其质量标准的研究提供依据。方法:采用高效液相色谱法。Wondasil C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸溶液梯度洗脱,流速1.0 m L·min~(-1),检测波长分别为325 nm(0~18 min,咖啡酸),330 nm(18~30 min,毛蕊花糖苷、异毛蕊花糖苷),311 nm(30~55 min,广藿香酮),柱温30℃。结果:广藿香配方颗粒中咖啡酸、毛蕊花糖苷、异毛蕊花糖苷和广藿香酮进样量分别在0.021 8~0.218μg(r=0.999 8),0.032 6~0.326μg(r=0.999 8),0.099 4~0.994μg(r=0.999 7),0.174 2~1.742μg(r=0.999 9)与峰面积呈良好的线性关系,供试品溶液中4种被测指标成分在24 h内稳定性良好,平均加样回收率分别为98.85%,99.53%,99.37%,100.58%,RSD分别为1.9%,1.1%,1.4%,2.0%。结论:该方法同时测定了广藿香配方颗粒中非挥发性成分咖啡酸、毛蕊花糖苷、异毛蕊花糖苷和挥发性成分广藿香酮的含量,方法可行,重复性好,准确度高,可以为广藿香配方颗粒的质量控制提供方法参考。
Objective: To establish a HPLC method to simultaneously determine the content of caffeic acid, acteoside, isoacteoside and pogostone in Pogostemonis Herba formula granules, in order to provide basis for studying its quality standards. Method: An HPLC method was developed. Wondasil C18 column (4.6 mm×250 mm, 5 μm) was used and eluted with mobile gradient of 0.1% phosphoric acid-acetonitrile at the flow rate of 1.0 mL·min-1. The wavelength were set at 325 nm (0-18 min, caffeic acid), 330 nm (18-30 min, acteoside and isoacteoside), and 311 nm (30-55 min, pogostone). The column temperature was 30 ℃. Result: The good linear relationships between the concentration and peak area were between 0.021 8-0.218 μg (r=0.999 8) for caffeic acid, 0.032 6-0.326 μg (r=0.999 8) for acteoside, 0.099 4-0.994 μg (r=0.999 7) for isoacteoside acid and 0.174 2-1.742 μg for pogostone (r=0.999 9), respectively. The four tested indicators of sample solutions were steady within 24 h. The average recoveries were 98.85%, 99.53%, 99.37%, 100.58%, with RSD of 1.9%, 1.1%, 1.4%, 2.0%, respectively. Conclusion: Caffeic acid, acteoside, isoacteoside and pogostone were determined by this method simultaneously. The method is feasible and accurate with a good reproducibility, and can provide a reference for quality control.
