[Objective]To explore the value of （1,3）-β-D-gluean（G test）, in bronehoalveolar lavage fluid （BALF） for the diagnosis of invasive pulmonary fungal infection（IPFI） in children. [Methods] A total of 135 pediatric patients hospitalized in our hospital were chosen and divided into IPFI group（ n = 34）, simple pneumonia group（ n = 53） and non-infection group （n = 48）. All patients underwent fiberoptic bronchoscopy. BALF was collected. Meanwhile blood samples were collected for serum G test. [Results] The G test levels in BALF and serum of IPFI group were 121±65.93 ng/L and 23.49±20.49 ng/L respectively, which were oh viously higher than those in simple pneumonia group（27.6 ±10.35 ng/L and 9.8 ± 7.62 ng/L, respectively） and non-infection group（10±-6.25 ng/L and 5±5. 12 ng/L, respectively）, and there were significant differences（ P 〈0.05）. The G test levels in BALF of IPFI group were obviously higher than those in serum of same group, and there was significant difference（ P 〈0.05）. The best critical value of G test in BALF for the diagnosis of IPFI was 42.02 ng/L. The sensitivity and specificity of G test in BALF for the diagnosis of iPFI were obviously higher than those of serum G test. [Conclusion] The G test in BALF for the diagnosis of IPFI has higher sensitivity and specificity than that in serum. The G test in BALF for distinguishing simple pneumonia and fungal infection has more advantages than serum G test, and it can reduce misdiagnosis and missed diagnosis, and provide the basis for the use of antifungal drugs, and decrease the mortality rate of IPFI in children.