Objective To investigate the level of hepcidin on the hyperlipidemia model induced by lipid emulsion, researching the relationship between liver function with changed hepcidin. Methods Aged 4 weeks sixty male C57BL/6J mice were randomly divided into control and model groups,with in 10per group. Expect that 10 mice as control group were sacrificed and 10 mice were intragastrically administered normal saline, others were intragastrically given Fat-emulsion respectively for 2,4,6,8 weeks to generate the hyperlipidemia with liver damage model. After 8 weeks, all established mice were anesthetized. Blood was collected in sterile tubes. Liver were removed and sacrificed by cervical dislocation. Serum lipids parameters were measured with an automated analyzer. hepcidin levels were determined using an enzyme linked immunosorbent assay. Results Compared with the control group, all treatment group TC/TG/LDL-C/hepcidin had significantly decrease(P<0.05),especially for 8-week group(P<0.01).From the pathology,we can observe the change with necrosis and steatosis(P<0.05). Conclusion The hepcidin protein may be inhibited by dysfunction of liver, comparative with the expression of hepcidin affect by liver function. All the results may give a direct evidence to differentiation of syndrome and provide new target to therapy and clinical laboratory and to access the prognosis of hyperlipidemia.