Broomrape occurrence on sunflower becomes a serious threat. To better understand the damage of broomrape parasitism in whole growth period of sunflower, root and shoot physiological characteristics, dry matter accumulation, yield and yield components in 3 different parasitism severity of sunflower broomrape were investigated in 2 sites. Cultivar SH363 was used as plant material. Results showed that broomrape parasitism significantly reduced sunflower yield. In light parasitism (L-H) treatment broomrape, yields were 3.66 t·hm-2 and 3.23 t·hm-2 respectively in 2 sites, declined 24.85% and 16.32% comparing with control yield. In heavily harm (H-H) parasitism treatment of broomrape, yields were 2.70 t·hm-2 and 1.98 t·hm-2 respectively in 2 sites, declined 44.56% and 48.70% comparing with control yield. Broomrape parasitism significantly decreased the number of harvest, full grain number per disk, full grain weight per disk, hundred-grain weight. Dry matter accumulation during anthesis and maturity was significantly decreased. Root biomass especially at 20-40 cm-soil layer quickly decreased during late growth period. Thus, root growth was limited, leaf area index and photosynthesis accumulation declined as the sunflower broomrape parasitizm damage increased, resulting in a lower grain yield.