知识与财富的链接
目的:建立藏药翼首草的高效液相色谱(HPLC)指纹图谱,并对不同产地翼首草的指纹特征进行比较,为其质量评价提供新的方法。方法:采用HPLC 法测定了10 批不同产地的翼首草样品。色谱柱：Boston Symmetrix(250×4.6mm,5μm),流动相：甲醇-0.1mol.L-1乙酸铵溶液(81:19),流速：0.8mL.min-1,检测波长：210nm,柱温：30℃。并对不同产地样品的相似度进行了比较。结果:利用《药典会指纹图谱相似度评价系统(2004A版)》建立了翼首草药材的指纹图谱共有模式,标出了11个特征峰。10批样品的相似度均较高。结论:本方法具有较好的重复性、精密度、稳定性,可为翼首草的质量控制提供科学依据。
Objective: To establish HPLC fingerprint of Tibetan medicine Pterocephali Herba, and compare it of Pterocephali Herba from different place, provided a new methods for its quality evaluation. Method: Adoption the method of HPLC to determine 10 groups of Pterocephali Herba from different place. Chromatographic Column was Boston Symmetrix(250×4.6mm,5μm), mobile phase was Methanol-0.1 mol.L-1 ammonium acetate solution(81:19),velocity was 0.8mL.min-1, the detection wavelength was 210nm, column temperature was 30℃. And compared the similarity of Pterocephali Herba from different place. Result: Taking advantage of < USP fingerprint similarity evaluation system (2004A version)> to establish common mode of HPLC fingerprint of Tibetan medicine Pterocephali Herba, and marked 101 characteristic peaks. The 10 groups samples had high similarity. Conclusion: This method had good repeatability, precision, and stability, providing scientific basis for quality control of Pterocephali Herba.
