知识与财富的链接
甜菜M14品系在细胞胚胎学和遗传学特征上具有鲜明的无融合生殖现象。为了寻找在这一特殊的生殖过程中的相关基因及其调控作用．实验通过同源克隆及cDNA末端快速扩增（RACE）的方法，首次从甜菜M14品系中克隆了与生殖相关的基因ByCK2（Beta vulgaris casein kinase2）的全长cDNA序列．长度为1501bp，开放阅读框为1002bp，编码333个氨基酸。根据氨基酸序列计算蛋白分子量为39．28kDa，pI=8．16。同源比对表明，ByCK2与烟草CK2（Ge．nBankNo．A1437635）的相似度为64.22％，与百合CK2（GenBankNo．AF517838）的相似度为65．18％。通过细胞内定位分析．ByCK2所编码的蛋白主要存在于细胞核中。
Beta vulgaris line M14 has an obvious characteristic of apomixis phenomenon in cell embryology and genetics. In order to find related genes in such process and its function, BvCK2 （Beta vulgaris casein kinase 2） gene was firstly cloned by using the method of homologous clone by the technique of Rapid Amplification of cDNA End （RACE）. The full-length cDNA sequence of BvCK2 is 1 501 bp in size and has an open reading frame of 1 002 bp encoding a polypeptide of 333 amino acids. The pI/protein molecular weight deduced from the amino acids sequence is 8.16 and 39.28 kDa. Homologous alignment indicates that it is 64.22% homology with tobacco （GenBank No. AJ437635）, 65.18% homology with lily （GenBank No. AF517838）. Prediction of protein localization sites （version6.4） indicates that the coding protein of BvCK2 gene mainly exists in nucleus.
