知识与财富的链接
以新选育的木荷（Schima superba）优良家系为试材,开展组织培养。结果表明,木荷适宜的初代培养基为GD+6-BA 1.50 mg·L-1+NAA 0.50 mg·L-1,继代培养基为GD+6-BA 1.00 mg·L-1+ IAA 0.30mg·L-1,生根培养基为1/4 MS+ IBA 1.00 mg·L-1+IAA 0.40 mg·L-1。芽苗增殖倍数为3.1倍,生根率达96.67％。探索出适宜的驯化、移栽和后期管理技术,使优良家系生根苗的移栽存活率达到92.2％。
A newly-selected superior family of Schima superba Gardn.et Champ was used for tissue culture and micropropagation. The explants of stem segments displayed the optimum initial growth on the primary media GD 6-BA 1.50 mg?L-1 NAA 0.50 mg?L-1, the highest proliferation rate of 3.1 times on subculture GD 6-BA 1.00 mg?L-1 IAA 0.30 mg?L-1, and 96.67% rooting rate on medium 1/2GD IBA 1.00mg?L-1 NAA 0.05mg?L-1 IAA0.80mg?L-1 Suitable techniques and procedures were identified for acclimatization, transplantation and later-period management of tissue-cultured plantlets. The survival rate of the superior family was 92.2%.
