知识与财富的链接
目的 运用代谢组学方法对黄芩素作用白假丝酵母菌引起的胞内代谢差异进行表征,寻找潜在的生物标志物并探讨相关的药物作用机制.方法 采用气相色谱质谱方法测定黄芩素作用前后白假丝酵母菌胞内的代谢指纹谱,利用多元统计分析方法比较黄芩素给药组与对照组的代谢物谱差异,筛选出可能的潜在生物标志物,并作出相关代谢途径的可能解释.结果和结论 经比较分析,共筛选得到20个潜在生物标志物,这些重要的差异代谢物主要参与了氨基酸代谢、三羧酸循环、磷脂代谢和氧化应激等相关通路.
Objective To conduct a metabolic analysis of the intracellular metabolism in baicalein-treated Candida albicans, so as to search for possible biomarkers and to discuss the mechanism of baicalein. Methods GC-MS analysis was used to obtain the metabolic fingerprint of C. albicans before and after treatment with baicalein, and multivariate statistical analysis was used to identify the differences in intracellular metabolites between the treated and control groups, so as to search for the possible biomarkers and discuss the possible relevant metabolic pathways. Results and conclusion Twenty metabolites were screened out and selected as potential biomarkers, and they were primarily involved in oxidative stress, citrate cycle, lipid metabolism and amino acid metabolism.
