知识与财富的链接
目的 建立市售啤酒中嘌呤含量的评价指标。方法 采用反相离子对色谱法对16种市售啤酒中嘌呤含量进行检测。结果 游离嘌呤碱基含量为5.72~26.52 mg/L,嘌呤核苷含量为40.14~135.56 mg/L,总嘌呤碱基含量为31.69~92.66 mg/L。游离嘌呤碱基和嘌呤核苷两部分之和与总嘌呤碱基含量间存在非常显著相关关系,相关系数为0.985(P＜0.01)。结论 以游离嘌呤碱基和嘌呤核苷为指标,可以满足不同啤酒间嘌呤含量的评价,检测方便快捷且能更好地反映啤酒中主体嘌呤物质。
Objective To establish a simple index for evaluating purine content in beers. Methods Purine contents in 16 comercial beer samples were determined by the reverse phase icon pair chromatography. Results The results showed that the content of free purine bases (TFP) was between 5.72 mg/L and 26.52 mg/L; the content of purine nucleosides (TN) was between 40.14 mg/L and 135.56 mg/L; and the total content of pu-rine bases (TP) was between 31.69 mg/L and 92.66 mg/L. There were significant correlations between two parts of free purine bases and purine nucleosides and the total contents of purine bases, and the correlation coefficient was 0.985 (P＜0.01). Conclusion The contents of free purine bases and purine nucleosides are determinated simply and rapidly, which can be satisfactorily applied to evaluate purine contents of different beers. And the indices can reflect the main purines in beers better.