知识与财富的链接
采用PCR-walking方法对水稻抗白叶枯病突变体G48的侧翼序列进行分析。通过特异性嵌套引物扩增和序列比对分析获得了该突变体T-DNA左、右边界侧翼序列,确定T-DNA插入水稻日本晴第三染色体clone OSJNBa0076E06(AC132215.1)位点上,左、右边界插入位点分别位于该克隆的第93 750、93 824碱基处。PCR-walking方法为T-DNA的侧翼序列分析提供了一种简单、高效的方法。
The flanking sequence of a rice T-DNA inserted mutant G48 with a blight bacteria resistance was analyzed via PCR-walking.Results showed the T-DNA was inserted into the clone OSJNBa0076E06 (chromosome 3,AC132215.1) in Oryza sativa L.japonica cv.nipponbare.The left and right border of T-DNA inserted was 93750th and 93824th nucleotide of OSJNBa0076E06,respectively.PCR-walking provided a simple and effective method for analyzing flanking sequence of T-DNA insertion.
