In order to alleviate the contradiction between supply and demand of water resources in Hotan region and meet the task of sluicing from Hotan to Tahe River, According to the hydrological data in Hotan River basin, this paper calculated the water loss rate along the river from upstream control station to downstream Xiaota station under three working conditions and analyzed the reasons of water loss along the two major tributaries in Hotan river. Through further correlation analysis, this paper got the critical water of downstream Xiaota station receiving from upstream, synthesis minimum discharge flow of canal head of Galakashihe to Yulongkashihe River and mimimum diacharge flow of Aigeliya and Dukeluke station. The results have a certain reference value for the development and management of water resources in Hotan River basin, and discharge flow to The Tarim River.