知识与财富的链接
以早熟禾(Poa pratensis cv.Merit)、黑麦草(Lolium perenne cv.Green Emerald)和白三叶(Trifolium repens cv.Riverdel)3种冷季型草坪草为材料,探讨2种典型融雪剂(1号融雪剂含NaCl 45.2%,2号融雪剂含NaCl 54.6%)对其种子萌发、幼芽和幼根生长的影响,并测定幼苗生长过程中含水量、质膜相对透性、丙二醛含量及过氧化物酶(POD)活性。结果表明:随着融雪剂浓度的增加,3种草坪草种子萌发和幼苗生长受到的抑制作用增强,2号融雪剂对草坪草种子萌发和幼苗生长的抑制作用比1号融雪剂强;3种草坪草对融雪剂胁迫的耐受能力大小依次为黑麦草、早熟禾、白三叶,耐受临界值分别为6.25~9.61、4.99~6.17和1.97~3.11 g/L,极限值分别为12.89~16.85、9.79~12.62和5.71~8.26 g/L;融雪剂胁迫主要通过渗透效应和离子毒害,抑制草坪草对水分的吸收,破坏质膜结构,增加MDA的积累,但3种草坪草幼苗的POD酶均能起到一定的保护作用。
The effects of two typical deicing salts (mass concentration of NaCl in No. 1 deicing salt was 45.2%, in No. 2 deicing salt 54.6% ) on seed germination and growth of shoots and roots of three kinds of cool-season turfgrasses (Poa pratensis cv. Merit, Lolium perenne cv. Green Emerald and Trifolium repens cv. Riverdel) were studied. The length of roots and shoots, relative water content, relative permeability of plasma membrane, malondialdehyd (MDA) content and peroxidases(POD) activity of turfgrass seedlings were also determined in this paper. The results showed that the inhibition effect on seed germination and growth response in the three kinds of turfgrasses was increased with increasing concentration of deicing salts. Compared with No. 1 deicing salt, the inhibition effect of No. 2 deicing salt was stronger. Lolium perenne showed the highest tolerance to deicing salts, followed by Poa pratensis and Trifolium repens. The critical value of tolerance to deicing salts were 6.25–9.61 g/L, 4.99–6.17 g/L and 1.97–3.11 g/L for Lolium perenne, Poa pratensis and Trifolium repens, respectively and the maximum value were 12.89–16.85 g/L, 9.79–12.62 g/L and 5.71– 8.26 g/L, respectively. The deicing salts mainly inhibited water absorption, disturbed membrane function and induced MDA accumulation via the effect of osmotic and ion toxicity, and POD enzyme of the three kinds of turfgrass seedlings provided some protection against the deicing salt stress.