知识与财富的链接
目的:建立HPLC同时测定粉葛饮片中葛根素、大豆苷元-8-C-芹菜糖-(1→6)葡萄糖苷和大豆苷3种黄酮类成分含量的方法。方法:采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.02%磷酸(25∶75)为流动相,检测波长250 nm。结果:精密度、稳定性和重复性合格,3个成分的线性范围分别为0.123～0.737,0.024 9～0.150,0.021 7～0.130μg,3个成分的平均回收率分别为99.4%,99.0%,99.1%,测定了4批粉葛饮片中3个成分的含量。结论:该方法简便、快速、准确度高,可为粉葛饮片的质量控制提供参考依据。
Objective: To establish a HPLC method for determination of puerarin, daidzein-8-C-apiosyl-(1→6) glucoside and daidzin in Radix Pueraria thomsonii. Method: The mobile phrase was methanol-0.02% phosphoric acid (25: 75) with a Diamonsil C18 column (4.6 mm×250 mm, 5 μm), detection wavelength at 250 nm. Result: The accuracy, stability, and reproducibility of this method all met the requirements.The linearity range of puerarin, daidzein-8-C-apiosyl-(1→6) glucoside and daidzin is 0.123-0.737, 0.024 9-0.150 μg and 0.021 7-0.130 μg.The average recovery of three components is 99.4%, 99.0% and 99.1%.The method was applied to determine the content of four batches of Radix Pueraria thomsonii. Conclusion: The method is rapid, simple and accurate, which can be used in the quality control in Radix Pueraria thomsonii.
