知识与财富的链接
利用ITS区和12S rRNA基因对广东徐闻地区7种滨珊瑚科珊瑚的遗传多样性和系统发生关系进行了研究。结果表明: (1) 基于ITS1检测到在58个样本中有48个单倍型, 多样性在0.800—1.000之间, 多态位点数在5—62之间, 核苷酸多样性指数在0.012—0.094之间; 基于ITS2 检测到43个单倍型, 多样性在0.742—1.000之间, 多态位点数在22—75之间, 核苷酸多样性指数在0.017—0.175之间。基于ITS区所得遗传分化系数在?0.00409—0.12170之间(P<0.001), 遗传分化不明显, 7种珊瑚中, 二异角孔珊瑚的遗传多样性最丰富。(2) 以12S rRNA基因建立的系统发育树与传统的形态学分类系统基本吻合, 7 种珊瑚被分成了3个支系, 滨珊瑚属3种为一个支系, 二异角孔珊瑚为一个支系, 其余3种角孔珊瑚为一个支系。
Seven species, 58 samples of Poritidae were selected, the sequence of ITS and partial 12S rRNA were get, to analyze their genetic diversity and phylogenetic relationship. (1) The results of the genetic diversity of the ITS1 shows, 48 haplotypes were detected, the haplotype diversity index was between 0.800—1.000, and the polymorphic loci is between 5—62. The nucleotide diversity index was between 0.012—0.094; the results of ITS2 show that 43 haplotypes were detected, haplotype diversity index was between 0.742—1.000. The polymorphic loci was between 22—75, and the nucleotide diversity was between 0.017—0.175. Among these seven species, the genetic differentiation index based on the ITS was between ?0.00409—0.12170 (P<0.0124), genetic differentiation was not obvious. Goniopora duofasciat has the highest genetic diversity. (2) The results of phylogenetic relationship based on the 12S rRNA is that 7 species of coral have been were divided into three branches in the phylogenetic tree, three species of Porite were in the same branch. Goniopora duofasciat was on separate branch, the other three species of Goniopora were on the other branch. The monophyly is relatively clear in the phylogenetic tree. Among the Porites genus,Porites lutea and Porites pukoensis got together, and Porites nigrescens was on the separate one. Goniopora gracilis and Goniopora minor got together, and Goniopora columna was on the separate one. The phylogenetic tree based on 12S rRNA basically consistent with traditional classification system.
