Taking the fruits of ＇ Garden blue＇ rabbiteye blueberry as test materials, we studied the effect of 0.5% chitosan （0.4% high-molecular weight chitosan ＋0.1% low-molecular weight chitosan） coating treatment on quality of blueberry during storage at room temperature. The results showed that, during the 20 days storage under the normal temperature of 20 ℃, the ehitosan coating treatment could effectively slow down the failing speed of sensory quality and fruit firmness of blueberry fruit, reduce the rot rate and weight loss rate, maintain a stable TSS and organic acid content, inhibit the rapid increase of PPO activity and the large accumulation of MDA, prolong the storage time at room temperature, and was good for maintain postharvest quality of blueberry. But the chitosan coating treatment had no obvious effect on the anthocyanin content, and had inhibitory effect on the increase of flavonoids content of postharvest blueberry fruit.