综述了应用于污染土壤微生物多样性分析的各种分子生物学方法 ,包括DNA中mol%G C丰度的分析、核酸杂交技术、DNA复性动力学研究及基于PCR技术的DNA指纹图谱分析等方法。阐述了这些方法的主要应用及各自的优势和局限性
Several molecular biotechniques which have been used for study on microbial diversity in polluted soils were reviewed. These approaches include analysis of mol% (G C) abundance of DNA,nucliec acid hybridisation,DNA reassociation kenitics and DNA fingerprints pattern based on PCR technique and so on. Both of the advantages and limitations of these approaches were also discussed.