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比色法测定Fenton反应产生的羟自由基

比色法测定Fenton反应产生的羟自由基

ISSN:1000-3282
1996年第23卷第2期
经验交流
贾之慎,邬建敏,唐孟成 Jia Zhishen,Wu Jianmin and Tang Mengcheng

Fenton反应产生的羟自由基能与水杨酸生成羟基化产物2,3-二羟基苯甲酸,用比色法测定其含量能间接测定羟自由基的生成量.通过对测定条件的研究,得到最佳的测定方案.可作为一种简便的筛选羟自由基清除剂的方法

Hydroxyl radicals from Fenton reaction can react with salicylate to form 2,3-dihydroxybenzoate.The quantitative analysis of hydroxyl radicals can be done through the colorimetric determination of 2,3 -dihydroxybenzoate.The determination condition has been studied and a best determination program has been obtained. The test indicated that hydroxyl radicals from this system could be scavenged by reutin and quercitin.This system can be used as a convenient method for the selection of hydroxyl radical scavenger.

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ISSN:1000-3282
1996年第23卷第2期
经验交流

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