A gene chip was developed by preparing highly conserved DNA fragments for five animal infectious disease viruses ( Foot and mouth disease virus, Vesicular stomatitis virus, Bluetongue virus, Epizootic haemorrhagic disease virus of deer, Akabane disease virus ) using molecular cloning methods, and the DNA fragments were spotted on to specially treated glass slides. RNA extracted from samples was reverse transcripted, followed by PCR amplification and labeling with fluorescent dyes. The labeled cDNA and prepared gene chip were subjected to specific hybridization, and the hybridization results were detected by photoscanning. The results showed that this gene chip technique could identify and distinguish the five animal infectious diseases viruses. This method is rapid, sensitive and specific, and can screen and quarantine a large number of animal samples within a very short period of time.