知识与财富的链接
以巯基丙酸(HS-CH2CH2COOH)为稳定剂,在水溶液中合成了具有窄而对称(FWHM=40nm)的荧光发射带且尺寸为3nm的CdTe半导体纳米粒子,并用此纳米粒子成功地标记了生物分子胰蛋白酶.与单独的CdTe纳米粒子的溶液相比较,CdTe-胰蛋白酶溶液的吸收光谱在400～600nm范围内较为平坦,其发射光谱蓝移8nm,但发射峰的半峰宽不变.实验证明,CdTe-胰蛋白酶溶液吸收和发射光谱的变化是由CdTe纳米粒子与胰蛋白酶之间的结合反应引起的,而不是由空气中的O2所引起的,加热可促进CdTe纳米粒子与胰蛋白酶之间的结合反应.
CdTe semiconductor nanoparticles were synthesized in aqueous solution by using mercaptopropyl acid(HS-CH 2CH 2COOH) as the stabilizer. The nanocrystals exhibit a strong, stable and narrow band luminescence(FWHM=40 nm). The average size estimated from TEM graph is ca. 3 nm. The applicability of the resulting mercaptopropyl acid modified CdTe nanoparticles to the labeling of biomoleculestrypsin was studied in this paper. The absorption spectrum of CdTe-trypsin solution was flatter than that of CdTe nanoparticles over the wavelength range of 400 to 600 nm. While the emission spectrum of CdTe-trypsin solution shows a blue-shift in the emission peak, the intrinsic emission band width is unchanged. It was proved that the shift of the emission peak was the result of the conjugation between CdTe nanoparticles and trypsin, but not of the oxidation caused by O 2. Furthermore, heating could accelerate the speed of conjugation between CdTe nanoparticles and trypsin.
