研究了沼泽红假单胞菌(Rhodopseudomonas palustris)S菌株的生长、Cd2 的去除与供氧光照、碳源、pH及温度的关系。结果表明:在好氧黑暗、苹果酸为碳源、pH7.0和30℃条件下,S菌株对初始浓度为25mg/L的Cd2 去除率最大,达到85%。在最佳条件下,Cd2 初始浓度为10mg/L~25mg/L时,去除率达85%,Cd2 浓度增加到150mg/L时,去除率仅为23%。测定Cd2 在菌体不同部位的分布发现菌体中富集的Cd2 63.5%在细胞壁上,33.5%在细胞原生质体内。同时,结合该菌体细胞超薄切片透射电镜观察证明了S菌株对Cd2 的去除主要在细胞壁上。
The influences of oxygen supply and illumination, carbon sources, pH and temperature on the growth of Rhodopseudomonas palustris S stain and removal for cadmium were studied. The results showed that the removal rate in presence of 25mg/L of cadmium was the highest, reaching 85%, under oxygen supply and dark, carbon source with malic acid, pH=7.0 and temperature at 30℃. Under the optimal condition, the removal rate reached 85% in presence of 10mg/L~25mg/L of cadmium, while it was only 23% in presence of 150mg/L of cadmium. The distribution analysis of Cd2+ in different fractions of cell showed that 63.5% of Cd2+ was on cell wall and 33.5% of Cd2+ was in protoplast. Simultaneity, transmission electron microscopy photos of cell ultra thin section revealed that the removal of cadmium ions was mainly completed on cell wall.