知识与财富的链接
利用自编的脉冲程序,采用预饱和和自旋锁定对水峰进行双重抑制的方法,得到了 15N标记蛋白GAL4(62)的2D 1H-15N HSQC、HSQC-NOESY、HSQC-TOCSY谱,并对这几个谱在蛋白质 1H谱的归属中所起的作用进行了讨论.
Using the pulse microprogram edited 2D 1H-15N HSQC, HSQC-NOESY, HSQC-TOCSY spectra of the 15N-labeled protein GAL4(62) were abtained, while solvent suppression was achieved by presaturation during the relaxation delay and by the use of spin-lock purge pulses. The important role of those 2D experiments in 1H assignments of 15N-labeled proteins was discussed.