The research on gamete fertility and its morphological variations in the offsprings of triploid Chinese white poplar [( Populus tomentosa × P. bolleana ) × P.tomentosa ] was conducted along with the reference parents of diploid poplars ( P.tomentosa × P. bolleana ) and ( P. alba × P. glandulosa ). The results show: ① the triploid Chinese white poplar (B312) produces larger quantity of pollen yield with the average of 53 mg per catkin. It is found that the trend of higher estimates for the gamete fertility was observed if the diploids were used as reference mother parents. ② Female gamete fertility of the triploid Chinese white poplars would be comparatively accurate if the diploids were used as reference father parents, of which the fertility ranging from 0.05%～0.5% for female triploid Chinese white poplars such as B303, B304 and B305. ③ Some morphological variations such as tricotyledons and quadricotyledons were observed in the hybrid offsprings between the triploid Chinese white poplars [( Populus tomentosa × P. bolleana) × P. tomentosa ] and P.tomentosa × P. bolleana , with a total variation rate of up to 26.47%. ④ A total of 170 hybrid seedlings were obtained from crossing among the triploid Chinese white poplar and diploid poplars, of which abnormal poplar seedlings accounting for 14.7% of total seedlings. The phenomena such as variations in the cotylodens and seedlings of offspring hybrids among the triploid and diploid poplars might be related to the equilibrium of cell chromosomes. There is no doubt that these poplar hybrids will play important roles on further research in poplar genetics and breeding.