知识与财富的链接
宿主细胞蛋白在HCV复制过程中起着重要的作用.应用蛋白质-核酸紫外交联法检测多个细胞株,目的是明确是否存在可与HCV复制中间体3′末端特异性结合的细胞蛋白质.结果显示,在多个细胞株中存在一个分子质量约为45 ku的蛋白质（命名为p45）,可与HCV复制中间体3′末端131～278 nt结合,这种结合可被过量的自身未标记RNA竞争抑制,而非同源蛋白和非同源RNA均不能竞争抑制这种结合.根据计算机RNA二级结构分析推测,p45可能特异性结合于HCV复制中间体3′端的一茎环结构内.这一结果提示,p45在HCV子代RNA复制中可能起着重要作用.
In order to study the possible mechanism of the cellular proteins involved in the process of replication of HCV RNA. UV cross-linking experiment was used to identify the cellular proteins which bind to the 3′-end of HCV intermediate negative-strand RNA. Results showed that a protein with approximate molecular mass of 45 ku(p45) cross-linked with the 3′-end 131～278 nt of HCV intermediate negative-strand RNA. The p45 presented in various cell lines of different origins. The amounts of RNA-protein complexes increased with increasing amounts of cellular extracts. Non-homologous proteins and RNA transcripts could not compete for the binding between p45 and the 3′-end of HCV intermediate negative-strand RNA. These results suggested that the cellular protein p45 can specifically bind to the secondary structure of the 3′-end of HCV intermediate negative-strand RNA, thus p45 may play an important role in HCV RNA replication.