Metallomics is an emerging and promising research field which has attracted more and more attentions. In this paper, the application of state of art analytical techniques capable of high-throughput quantification, distribution, speciation, identification, and structural characterization for metallomics are critically reviewed. High-throughput quantification of multielements can be achieved by ICP-MS （Inductively Coupled Plasma Mass Spectrometry） and NAA （neuron activation analysis）. The high-throughput multielements distribution mapping can be performed by fluorescence-detecting techniques such as SR-XRF （Synchrotron Radiation-X-ray Fluorescence）, XRF tomography, EDX （Energy Dispersive X-ray Fluorescence）, PIXE （Proton Induced X-ray Emission）, LA-ICP-MS （Laser Ablation-ICP-MS）, and ion-detecting-based SIMS （Secondary Ion Mass Spectrometry）. All the techniques for metallome structural characterization are generally low-throughput, such as XAS （X-ray Absorption Spectroscopy）, NMR （Nuclear Magnetic Resonance） and SAXS （Small Angle X-ray Scattering）. If automation of arraying small samples, rapid data collection of multiple low-volume and low-concentration samples together with data reduction and analysis is developed, high-throughput techniques will be available and in fact have partially been achieved. Further, bioinformatics is an important tool in metallomics study.