知识与财富的链接
目的 探讨经RGD多肽功能化后的微流控芯片系统识别/捕获肿瘤细胞的效果.方法 采用化学共价连接的方法固定RGD多肽,建立由流体控制系统、免疫反应系统构成的细胞识别芯片系统,用于细胞的筛选/捕获;选用不同密度的细胞进行进样试验,考察不同细胞密度对通道内细胞数量的影响,并讨论不同的孵育时间对细胞捕获的影响.结果 随着进样细胞密度的增加,进入通道内的细胞明显增多;此功能化后的芯片系统在15 min内对A549的捕获率为90%,在20min内可完全将细胞捕获于管壁.结论 经RGD多肽修饰的微流控芯片系统可在短时间内对A549细胞进行有效的筛选,且在细胞数量很低时,也能对其进行捕获.
Objective To evaluate the ability of bio-functionalization microfluidic-chip with RGD peptide to capture lung tumor cells(A549). Methods RGD peptide were through direct covalent linkage attached on micro-channel surface with 1-ethyl-3- 3-dimethylaminopropyl carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to A549 cells. Different cell densities and incubation time were employed to calculate the rate of captured cell by counting cells in the whole micro-channel. Results With the increasing sample density, the number of cells enhanced obviously in the micro-channel. As incubation time extended, the capture cells also increased. When the incubation for 15 min, the capture rate was about 90, while for 20 min was 100. Conclusion This bio-functionalization microfluidic-chip can capture lung cancer cells in a short time. Even though the density of cells is low, this chip can also capture the cell.
